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After allowing 8 hours for cell attachment, cultures were inoculated with HSV-1 viruses using the conditions defined in each Figure Legend and Results sub-section.
Table 2 compares the fixation probabilities calculated by the model presented here and those from Jansen et al. [ 22] using the conditions defined in the prior report, which includes the introduction of a single infected female into a population size of 100 and perfect CI.
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It was used under the conditions defined by Magwere et al. [12].
Using the experimental conditions defined in the optimization, the method allowed iron determination with achieved detection limit of 2.3 μg l−1 and precision (assessed as the relative standard deviation) of 9.3 2.8% for iron solutions of 10.0 150 μg l−1.
Using the experimental conditions defined in the optimization, the method allowed nickel determination with achieved sampling rate of 25 samples per hour, detection limit (3 s) of 2 μg l−1 and precision (assessed as the relative standard deviation) of 8.2 2.6%, for nickel solutions of 10.0 200.0 μg l−1 concentration, respectively.
These modules allow making GFP constructs with or without a signal peptide and/or a His tag Restriction-ligations were performed using the optimal conditions defined previously (Promega ligation buffer, and restriction-ligation followed by heat inactivation).
The left panels (a,c) show CNN histograms using the condition that cell-cell contact is defined by just one common element-element interaction.
The right panels (b,d) show CNN histograms using the condition that cell-cell contact is defined by at least two common element-element interactions.
One unit of β-xylosidase or β-glucosidase activities was defined as the amount of enzyme releasing 1 µmol of ρNP per minute using the defined conditions.
Using the defined conditions offered by this culture system, dose-specific, inhibitory effects of inflammatory cytokines on myelin formation were demonstrated, unequivocally.
Despite large numbers of naturally MAP infected animals, elucidation of host-pathogen interactions in the early phase of the disease is only possible using the defined conditions and variables of experimental animal models.
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