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In another reaction, forward and reverse Clost primers were used using the conditions for Firm internal primers.
Plasmid DNA (5 μg of each), including PBase and PB-hMyoD, was transfected with an NEPA21 electroporator (Nepagene) using the conditions described in Table 1.
Quantitative analysis of Mn, Cu, Co, Cd, Ba, Na, Mg, K, Ca, Fe, Ni, Zn, and As were performed using a PerkinElmer NexION 2000 ICP-MS (PerkinElmer Inc). using the conditions shown in Table S2.
The geometry of both heat exchangers was optimized using the conditions of thermolators in LNG regasification systems.
Moreover, using the conditions obtained, we establish a method for design of a H∞ state feedback controller.
Using the conditions of Theorem 3.1, we have (3.9).
Using the conditions which satisfy, it gives (2.18).
Using the conditions described in the Experimental Sect.
By using the conditions (i)–(iii), we obtain (3.10).
Using the conditions (C1) and (C2) and AKTT-condition of, we have (3.32).
A validation was performed using the conditions predicted by the model.
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