Microvessel density (MVD), total vascular area (TVA), and several other size- and shape-related parameters were quantified in the region of most intense vascularization as well as within and outside the PCs, using the computerized image analysis software Image Pro software v5.1 (Media Cybernetics Inc). on a Pentium III PC, as described previously [ 27].
The relative intensity of each protein blotting analysis was measured using a computerized software program (Bio-Rad, CA, USA), and the expression of activated and total VEGFR-2 were normalized with total VEGFR-2 and β-actin bands, respectively, to compare the expression of proteins in different treatment groups.
Relative densitometry was determined using a computerized software package NIH Image 1.63.
A physician, masked to treatment group, measures JSW using automated computerized software.
A 2-way repeated measures ANOVA (supplementation protocol × time) was used to assess differences in LDH, E2 concentration, testosterone concentration, protein levels and the linear 2−δδCT data set for gene expression measured with RT-PCR using computerized software (Statistica, Statsoft).
All statistical testing was completed using computerized software (SAS v9.1, SAS Institute, Cary, NC).
Randomisation will be performed using computerized software on a 1 1 basis.
Interstitial collagen volume fraction from the remote area was measured from picrosirius red-stained histological sections by light microscopy (×200) using computerized software (ImageJ, NIH).
Cosmetic outcomes were assessed by a three-member panel using computerized software Breast Cancer Conservative Treatment cosmetic results (BCCT.core) and by patient self-evaluation, including patient satisfaction.
