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The pooled PCR products were subsequently analysed on the GS20 platform using the complete sample preparation and analytical process, as recommended by the manufacturer (Roche).
Using the complete sample (n = 5,303), characteristics of informal caregiving were examined using frequencies and logistic regression analyses adjusted for age and gender.
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We could not use the complete sample at follow-up (N = 582) for the longitudinal analyses, since half of the sample took part in an intervention to reduce gender violence.
From a phylogenetic analysis with the ompA gene sequence sample CT-37 was genotyped as serovar C or K (data not shown) but from a phylogenetic analysis using the complete genome, sample CT-37 clustered with the urogenital serovars (D-K) rather than the ocular serovars (A-C).
Both sets of results were obtained following missing data imputation (note that web table 1 shows unadjusted results using the complete case samples).
All parent and teacher questionnaires were used in the complete sample.
The tests were performed using the complete set of observations sampled from the MODIS-based composites (in total 1085 pixels extracted from each band).
The mean square error when using the complete set of M samples, y = h + w ∈ R M, is constrained by the Cramér-Rao bound[39].
In addition, we computed Spearman rank correlation coefficients in a pairwise manner among all 23 RNAseq samples using the complete normalized FPKM data.
Using the complete MLST profile of all 434 samples of these 206 women, 126 unique STs could be identified of which 51 were novel to the C. trachomatis MLST database (http://mlstdb.bmc.uu.se).se
Another way to speed-up the pairing phase is to quickly estimate the fragment size distribution using a large-enough sample instead of using the complete set of aligned reads, and then apply this estimate to calculate the mismap probabilities.
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