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Neighbor joining tree was generated using the complete deletion method in MEGA4 program [56], [57].
The Maximum Parsimony trees were calculated using the complete deletion option, all codon positions and a CNI level of 3 with an initial tree by random addition of sequences (10 replicates).
Phylogenetic relationships among H2A.Z promoter regions were reconstructed using the neighbour-joining method using uncorrected nucleotide p-distances using the complete deletion option.
A neighbor-joining tree was conducted in MEGA 4 [ 74] using the complete deletion of ambiguous data and the maximum composite likelihood option.
Sites evolving under positive selection were searched for using the codon-based model implemented in CODEML in the PAML analysis package [ 51] using the "complete deletion" option, i.e. only sites with no indel in any of the sequences were included in the analysis.
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All locations having gaps and missing data were removed from the data set using the complete-deletion option.
Distances were estimated using the complete-deletion option in all cases and standard errors were calculated by the bootstrap method (1000 replicates).
Genes required for growth in different environmental conditions were identified in two systematic genetic screens [30], [31] using the complete set of viable haploid gene deletion strains [1].
Neighbor-Joining, Minimum Evolution and Maximum Parsimony phylogenetic analyses were performed on amino-acid and nucleotide sequences of various datasets (six LGR proteins with or without related proteins identified by BLAST; either whole proteins or their LRR or non-LRR domains) with the same software using p-distance and the complete deletion option.
The phylogenetic trees for each gene family were reconstructed by using the neighbor-joining (NJ) method [ 27], the complete deletion option was used to exclude any site which postulated a gap in the sequences.
Tree construction was achieved by the neighbor-joining method with the complete deletion option using the Jukes-Cantor matrix for nucleotide sequences and the PAM (Dayhoff) matrix for protein sequences, respectively.
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