Sentence examples for using the compatible restriction from inspiring English sources

The mouse Nat2 open-reading frame [22] was sub-cloned into pET28b (Novagen) using the compatible restriction enzyme sites, NdeI and EcoRI.

Make sure you use the compatible cable for your device.

Bait and prey vectors used were pGBKT7 and pGADT7, and cDNAs were cloned into the vectors using compatible restriction sites within the vector and contained within the primers.

The Fc regions of the immunoglobulin sequence were fused to MDs either by splicing by overlap extension PCR or by using existing compatible restriction sites [ 12].

This was implemented by the creation of a novel mammalian expression vector in which either scFv or Fc domain could be easily switched using compatible restriction sites.

The resulting fragment was ligated into the pJNTmcs (Meijnen et al. 2008) vector using the restriction sites MluI and Kpn2I (compatible with AvaI).

For two SNPs to be mutually compatible, all four primers must be pair-wise compatible using the selected thresholds.

Use the packages for restrictions.

We used the strategy of compatible cohesive end of restriction enzymes, such as Avr II ligated with Nhe I, to link various comportments to avoid restriction sites remaining on the vector after ligation.

Illumina-compatible libraries were prepared using the above procedure.

Firstly, they depend on the availability of unique and compatible restriction sites in the vector and the DNA fragment(s) to be cloned.