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Data was analysed using the comparative relative quantification method and samples were normalised to both Actin42A and GAPDH.
Analysis of resultant crossing thresholds (CT) was performed using the relative comparative CT method.
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The relative amount of each mRNA was calculated using the second derivative comparative Ct method.
The data were then quantified using the comparative Ct method for relative gene expression compared with GAPDH as endogenous control.
Real time PCR was used for relative quantitative comparative expression analysis in various stages of flower and fruit development in these two cultivars.
The target gene expression levels were determined by relative quantification using the comparative CT method, also known as 2-∆∆CT method (comparative 2 [delta][delta]Ct method).
Gene expression profiling was completed using the comparative Ct method of relative quantification.
The levels of mRNAs were quantified using the comparative CT method relative to levels of hypoxanthine phosphoribosyltransfease (HPRT).
Data was analyzed using the Comparative Ct method (ΔΔCt) method for Relative Quantification (RQ).
For both types of gene expression the target genes were quantified by relative quantification using the comparative CT method.
Gene expression patterns for the six selected mRNAs were calculated using the comparative crossing threshold method of relative quantification (ΔΔCq method) [26], and presented as relative (ΔCq) and fold change (FC) values.
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