Sentence examples for using the comparative quantification from inspiring English sources

Threshold cycle (Ct) for the gene of interest in both the test samples and control sample were adjusted in relation to a reference gene using the comparative quantification algorithms-ΔCt (Table 1).

The results were analyzed with the software provided with the instrument, using the comparative quantification function.

Expression levels of genes of interest were calculated relative to ACTIN using the comparative quantification analysis method (Rotogene-5; Corbett Research), which takes into account the amplification efficiency of each primer set.

Relative gene expression was calculated using the comparative quantification option of Rotor Gene 6000 software 1.7.

Fold changes were calculated by using the comparative quantification application of the RotorGene 6000 software.

Relative expression levels were determined using the comparative quantification feature of the Rotorgene software.

Data was analysed using the comparative relative quantification method and samples were normalised to both Actin42A and GAPDH.

The quantification analysis for target gene expression was performed using the relative quantification comparative CT method [ 9].

To perform the relative quantification using the comparative Ct delta method (Relative Quantification of Gene Expression, bulletin 2, Applied Biosystems), three independent reactions for each gene were done in parallel with the reference gene 18S.

Ribosomal protein 23 and elongation factor 1-beta expression levels were used as normalization controls using the comparative method of relative quantification [ 91].

These parameters were derived from the Global Burden of Disease 6 or, in the case of heavy alcohol use, the Comparative Quantification of Health Risks for 2004.