Proto-Mayan (the common ancestor of the Mayan languages as reconstructed using the comparative method) has a predominant CVC syllable structure, only allowing consonant clusters across syllable boundaries.
Modern Mayan languages descend from Proto-Mayan, a language thought to have been spoken at least 5,000 years ago; it has been partially reconstructed using the comparative method.
The altered transcripts were quantified using the comparative method.
Using applied phylogenetics, students observed the frequency of evolution of traits using the comparative method.
Some examples of computational method using the comparative method are miRScan, 2, 18 miRAlign, 19 ERPIN, 20 and microHarvester.
The related gene expression was calculated using the comparative method for the relative quantity normalized to GAPDH gene expression.
Relative quantification was determined using the comparative method: 18S ribosomal RNA was used as the housekeeping gene (Mm04277571_s1).
One of the most powerful indirect techniques in evolutionary biology is using the comparative method to investigate convergent evolution [ 83].
Data was analyzed using the Comparative Ct method (ΔΔCt) method for Relative Quantification (RQ).
Gene expression levels were normalized to Gapdh and calculated using the comparative CT method (ΔΔCT method).
