Sentence examples for using the comparative critical from inspiring English sources

Samples and controls were run in triplicate in separate wells, and the levels of gene expression were analyzed quantitatively using the comparative critical threshold (Ct) method.

Samples and controls were run in triplicate in multiplexed wells, and levels of gene expression were analyzed using the comparative critical threshold method.

Amplification data were analyzed using the comparative critical threshold (2-ΔΔCT) method [72].

The relative mRNA abundance was determined by using the comparative critical threshold method and is expressed relative to the control diet group [96].

Using the comparative critical cycle (Ct) method and using GAPDH as the endogenous control, the expression levels of the target gene products were normalized and the relative abundance was calculated.

Amplification conditions consisted of initial 12-min activation at 95°C followed by 40 cycles of denaturation at 95°C for 30 s, annealing at 58°C for 30 s and extension at 72°C for 30 s. Relative quantification of the PCR amplification products was performed using the comparative critical threshold (CT) method.

Quantification of relative mRNA abundance based on critical threshold (CT) was assessed using the comparative CT formula, 2-ΔΔCT, with GAPDH mRNA as an internal control.

Relative quantitation was performed using the comparative CT (∆∆CT) method53.

Relative gene expression was normalized to the Gapdh gene expression using the comparative Ct method.

Relative mRNA expression of target genes was normalized to ATCB using the comparative Ct method.

qRT-PCR data were calculated using the comparative Ct method (Life Technologies).