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Samples were compared using the comparative Ct method.
The real-time value for each sample was averaged and compared using the comparative CT method.
Expression values were calculated using the comparative Ct method.
Quantification was calculated using the comparative Ct method.
Quantification was performed using the comparative Ct method (2−ΔΔCt).
Data were analyzed using the comparative CT method.
Data were analyzed using the comparative CT method using CFX software (BioRad).
Data was analyzed using the comparative CT method following the manual described (Applied Biosystem).
The relative abundance of each miRNA transcript was then determined using the comparative Ct method [28].
Results were analyzed using SDS 2.2 software and relative expression calculated using the comparative Ct method.
Gene expression profiling was completed using the comparative Ct method of relative quantification.
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