Sentence examples for using the commercial kit from inspiring English sources
Spoligotyping was carried out by using the commercial kit from Isogen Bioscience BV (Maarssen, The Netherlands) [ 10].
Medium was collected at different times after OGD and subjected to ELISA, using the commercial kit Quantikine® for SDf-1α (R&D system, Milan, Italy).
Reverse transcriptase reactions were performed using the commercial kit High-Capacity cDNA Reverse Transcription (Applied Biosystem) and contained up to 2 µg of total RNA.
PCR amplicons were analyzed by agarose gel electrophoresis, extracted and purified using the commercial kit SV Gel and PCR Clean-Up System (Promega, USA).
Levels of TNF- α were measured using the commercial kit (eBioscience, Frankfurt, Germany) according to the manufacturer's protocol.
The DNA extraction was performed using the commercial kit MagneSil™ (Promega Corporation, Madison, Wisconsin, USA) following manufacturer instructions described elsewhere.
Glutathione transferase (GST) action was measure using the commercial kits provided by Bioassay Internationals.
GPx and SOD activities were achieved using the commercial kits Ransel and Ransod, respectively (Randox Laboratories Ltd., Crumlin, UK) according to the manufacturer's instructions.
Serum samples were analyzed for activities of antioxidant enzymes including GSH-Px and for TBARS using the commercial kits provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, China) [ 20].
Alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and bilirubin in serum samples were measured using the commercial kits of Merck, Mumbai, India.
Concentrations of E2 and FSH were determined by conventional chemiluminescent microparticle immunoassay using the commercial kits Architect Estradiol and Architect FSH from Abbot Laboratories (Abbott Park, IL, USA), respectively, according to the manufacturer's guidelines.
