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In order to compare TDFs between the incompatible and compatible reactions, all 186 uniseqs from the previous compatible interaction [ 34] were formatted to form one local compatible database using the command of "formatdb" in the BLAST system.
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When a dog jumps on you or the sofa, use the command "off" instead of "down".
The interpolated values have been obtained using the command pchip of the R package Signal, which uses the methodology described by Fritsch and Carlson (1980).
Profiles were used subsequently to screen the antennal and maxillary palp transcriptome database of L. y-signata using the command line version of HMMER.
The putative SNPs were then filtered against intron-exon junction positions using the command line version of Intron Finder software at Sol Genomics Network (SGN) website.
In the submitting test, the description file is shown in Figure 9. Firstly, we submit the task by using the command globusrun-ws of GT's command line tool.
Markers were assigned to intervals between two anchor markers using the command buildfw with LOD score of 3.
We tested for the presence of collinearity using the command _rmcoll in Stata.
RMA normalization [ 42] was performed for all samples together using the command-line utility of RMAExpress (http://rmaexpress.bmbolstad.com).
These XML BLAST data were then processed using the command-line version of blast2go (b2gPipe) with database version 2.3.5.
Ab initio gene prediction was performed using the command-line version of Augustus 2.3 software http://augustus.gobics.de/ using A. thaliana as plant model.
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