All sequences were annotated using Blastn and the combined database of A. thaliana and A. lyrata coding sequences (see Additional file 4: S1 and Additional file 5: S2 for a list of annotated genes).
ProteinPilot (version 4.5, AB Sciex, Foster City, CA, USA) was used to perform the combined database searches with all bands from a single lectin and secretion.
Next, the MS/MS spectra were searched against the combined database using pFind studio (version 2.8).
We then ran BLASTP on the low-stringency conopeptides against the combined database, using the following parameters: hitdist = 40 wordmask = seg postsw matrix = BLOSUM80.
The indices computed from the bootstrap sample represented the apparent performance, and those computed from the combined database using the β-coefficients from the bootstrap sample represented the test performance.
The blueberry cDNAs were used as queries in a BLASTX search of the combined database.
Heuristic parsimony analyses using the combined rbcL and trnL-F database were performed separately for every unknown gametophyte by including one individual at a time in the analysis.
Database searches were performed in PubMed and the Cochrane database by using the combined medical subject headings for 'right ventricular dysfunction or right ventricular dilatation' with the exploded term 'acute pulmonary embolism' and by scanning references in retrieved articles and reviews.
The protein database searches, using the combined PMF and MS/MS datasets, were performed using Bio Tools 3.2 software (Bruker).
Databases were searched using the combined medical subject headings for right ventricular dysfunction or right ventricular dilatation with the exploded term acute pulmonary embolism.
