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We then performed in vitro fertilization using the collected sperm to obtain mutagenized F1 fry.
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After gonadotropin treatment in each family, the collected sperm was used for in vitro fertilization.
For comparison, IVF data was obtained using freshly collected sperm from 847 GM lines being maintained on the same genetic backgrounds (Table S2).
The researchers collected sperm from the male after 1 week.
To study the sperm mitochondrial function, 0.5 mL of the newly collected sperm suspension was centrifuged at 1000 rpm for 2 minutes to remove the supernatant.
To see whether weight loss would affect sperm epigenetic markers, the researchers collected sperm samples from six men who were about to undergo gastric bypass surgery.
Comparable rates of fertilization were observed for most genetic backgrounds whether cryopreserved or freshly collected sperm was used.
We collected sperm from spawning male colonies using 60 mL and 500 mL plastic syringes, aiming for areas in the water column near spawning colonies where sperm density was visibly high, such as in the valley between two spawning pillars.
To determine whether the requirement for a pre-incubation was linked to limited concentrations of viable sperm or increased concentrations of dead sperm, freshly collected sperm were a) diluted to limit the number of live sperm or b) were mixed with dead sperm to simulate the proportion and concentration of dead sperm present following cryopreservation and thawing.
Freshly collected sperm samples were analyzed.
These species use a variety of methods for sperm transfer; for example, in some the females collect sperm released into the water, while in others the males have a penis that inject sperm into the female.
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