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Multiple-sequence alignments and phylogenetic trees were generated by using the CLUSTALW software (Figure S3).
Multiple alignments of the concatenated spacer sequences were carried out using the CLUSTALW software [40].
Sequences were aligned and analyzed using the ClustalW software [46] to determine polymorphism and synonymous or non-synonymous mutations.
The multiple sequence alignment of FadD13 with homologous proteins of Fatty Acyl-CoA Synthetase family was generated by using the ClustalW software [48].
Partial sequences of estrogen receptor genes from various vertebrates were aligned using the ClustalW software available on the web (http://bioinfo.hku.hk/services/analyseq/cgi-bin/clustalw_in.pl).
The protein sequences retrieved from Genbank (Genbank accession numbers of these sequences are given in Figure 7 and Figure 8 as well as those from pNH4 plasmid were aligned using the ClustalW software, version2 (http://www.ebi.ac.uk/Tools/clustalw2/index.html).html
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All sequences for the atpD (642 nucleotides), dnaX (536 nucleotides), gyrB (745 nucleotides) and recA (425 nucleotides) genes were edited and aligned using the ClustalW 1.6 software with parameters as: Gap Opening Penalty of 15, and Gap Extension Penalty of 6.66 for Pairwise and Multiple Alignment; Transition Weight of 0.5; and Delay Divergent Cut-off of 30%.
We performed sequence comparisons using the Clustalw v. 1.8 software package [ 60] and PipMaker [ 61].
Multiple sequence alignments of deduced amino acid sequences were made using the ClustalW multiple-alignment software (http://www.ebi.ac.uk/clustalw/index.html).html
Multiple sequences of each chimeric chain were aligned by frame regions (FRs), complementarity-determining regions (CDRs), and human constant genes (HcG1 or HcK) using the ClustalW multiple sequence alignment software, Discovery Studio 2.5 (Accelrys) (Fig. 2).
These partial sequences were aligned by using the ClustalW algorithm in BioEdit software v7.2.0.
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