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Transposase protein sequences were aligned using the ClustalW method [22].
The sequences were aligned with previously published OPV sequences from GenBank using the ClustalW method, and were manually aligned using MEGA software version 3.1 (Arizona State University, Phoenix, AZ, USA).
Full-length S- and L-segment nucleotide and amino acid sequences of the new hantavirus, amplified from the European common mole, were aligned with publicly available hantavirus sequences, using the ClustalW method (Lasergene program version 5, DNASTAR, Inc., Madison, WI) [30].
Amino acid or nucleotide sequences were aligned using the ClustalW method in MEGA 4 software [ 35].
Sequences were aligned by using the ClustalW method (http://www.genome.jp/tools/clustalw/). Trees were constructed by using the maximum-likelihood method and analysis with 1,000 bootstrap replications.
Predicted amino acid sequences for eae and cdtB were aligned with reference alleles by using the ClustalW method and MegAlign software (DNASTAR, Madison, WI, USA).
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Both nucleotide and predicted amino acid sequences were aligned by using the ClustalW 1.4 method (http://www.ebi.ac.uk/clustalw/).ac.uk/clustalw/
Sequences were aligned with published OPV sequences in GenBank by using the ClustalW (www.clustal.org/) method and manually aligned by using MEGA version 4.0 (Arizona State University, Phoenix, AZ, USA).
We aligned PEDV sequences by using the ClustalW (http://www.clustal.org/clustal2/) method.
An unrooted phylogenetic tree was constructed using the ClustalW program and the neighbour-joining method in the MEGA5 software package.
Because both methods resulted in consistent alignments, we continued using the ClustalW results for the sake of simplicity.
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