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The regions were aligned using the ClustalW alignment program (reference) with the default parameters.
The sequences were downloaded from Genbank and aligned using the CLUSTALW alignment of the amino acid sequences.
Open reading frames were translated into amino acid sequences and aligned by using the ClustalW alignment option in Geneious version 6.0.5 (www.geneious.com/).
Click "OK" and MEGA 4 will align the sequences you selected using the ClustalW alignment algorithm (alignment places highly similar, conserved regions in vertical columns by inserting gaps between and around them. Note: the gaps show up as hyphens).
The reverse complement of each miRNA was then aligned with its validated target regions using the ClustalW alignment package [ 25].
Clone sequences thus obtained were compared to the expected sequence using the ClustalW alignment algorithm [21].
Similar(44)
Using the ClustalW alignments and the nucleotide clusters, RevTrans was used to create codon-aware DNA alignments [ 71].
The C+ and C- multiple alignments were combined using the ClustalW profile alignment function and the resulting alignment was hand edited to correct poorly aligned regions.
Selected amino acid sequences of catalases were aligned with catalase LlonKat1 using the ClustalW Multiple Alignment tool in BioEdit Sequence Alignment Editor (http://www.mbio.ncsu.edu/BioEdit/BioEdit.html).html
The two groups of sequences were aligned using the ClustalW [50] alignment option available in the MEGA 4 program [51].
Sequences in the largest japonica rice subfamily were aligned using the ClustalW multiple alignment program.
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