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Primers and probe were designed by using the chlamydial sequences available in GenBank and were evaluated with Primer3 [ 17].
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Draw squares using the Fibonacci sequence.
Using the wrong operating sequence.
They used the bathrooms in sequence.
Chlamydiae membrane proteins were collected from all 7 chlamydial proteomes using the Polyphobius predictor algorithm [ 33].
Serum samples were tested for the presence of chlamydial antibodies using the CHECKIT™ Chlamydia Test Kit (Idexx GmbH, Ludwigsburg, Germany) according to the manufacturer's instructions.
The fraction of chlamydial sequences in the total DNA ranged from 4% (CWL011) to 32% (PB1).
Infectivity was calculated as the number of chlamydial inclusions per cell using the Scan R-Analysis software.
Pair-wise alignments of sequences for determining percentage identity between Chl. trachomatis proteins and those from other chlamydial species were carried out using the ALIGN Plus 4 program package (Scientific and Educational Software) using the BLOSUM 62 scoring matrix [ 79] and the default parameters of the alignment program.
Accordingly, the aim of this study was to estimate the chlamydial sero- and antigen prevalence in sheep flocks using the state of Thuringia as a model example.
Using the rapid antigen detection assay, 2 women were diagnosed with chlamydial infection compared with 6 infections detected by PCR.
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CEO of Professional Science Editing for Scientists @ prosciediting.com