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Using the challenge of counting the number of copies of kinetochore proteins in budding yeast as a case study, we discuss the basic considerations in developing a technique for the accurate evaluation of intracellular fluorescence signal.
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"I started thinking that I could use the challenge of doing it all myself," he says.
"To use the challenges of coalition, compromise politics as a stick to beat us with is turning facts on its head to put it mildly.
Even more, they want to use the challenges of returning the dolphins to an ocean setting to teach the public about the lives of, and threats to, their wild cousins.
Use the challenges of your business today to build mastery in your craft.
Bonobos has used the challenges of the current economic environment and the inherent limitations of a cash-strapped start-up (e.g., no marketing budget) to its advantage.
"Easy" might be a stretch, but once local riders get used to the challenges of Manhattan cycling, I'll grant that to the commissioner as well.
I used to enjoy the challenge of a good political argument.
"I used to enjoy the challenge of building things up from nothing," he said, but it gave him fits.
C&RT is one of many statistical tools that can be used to address the challenge of multipollutant exposures.
This analytic approach was used due to the challenge of interpreting clustering results when more than one covariate was included.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com