Exact(8)
Transfection of NB4 cells was performed using the cell line nucleofector kit V (Lonza, Basel, Switzerland).
First, the ability of ChoKβ-transfected cells to promote anchorage independent cell growth was determined using the cell line HEK293T.
The peptide was also tested using the cell line (LNCaPLN3), which was active in all assays and (PC3M), which was active in migration but not in proliferation.
Transient transfection of THP-1 cells with the miR-346 mimic (200 nM) or with miR-346 mimic and siRNATTP was performed using the Cell Line Nucleofector Kit V from Amaxa.
Taken together, these findings led us to analyze the influence of Krm2 on Wnt signaling in osteoblasts, which was first done in vitro using the cell line MC3T3-E1, weere we did not observe endogenous expression of Krm2 (Figure 1B) and Dkk1 (data not shown).
SNV verification was performed using the cell line derived DNA without sequence capture enrichment.
The relative mRNA expression was calculated using the cell line MCF7 as reference.
Therefore, we developed a claudin-low centroid-based predictor using the cell line database of Neve et al. [ 21].
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