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In-vitro mutagenesis by PCR-mediated recombination44 was performed as detailed before19, 20, 45 using the cDNA of ggTas2r1 cloned in the vector pcDNA5/FRT (Invitrogen) available from previous experiments9 as template.
Using the cDNA of pistils of tested pear cultivars as template and Cy3 fluorescently labeling primers by PCR amplification, microarray hybridization detected the S-genotype of each pear cultivar.
Using the cDNA of the putative receptors as a probe, a novel receptor was then cloned by hybridization from a MIN6 cDNA library which was generated from a mouse insulin-secreting cell line.
Using the cDNA of lung adenocarcinoma tissue as driver and its adjacent nonmalignant lung tissue as tester, the RSL which represented down-regulated transcripts, was constructed.
Using the cDNA of lung adenocarcinoma tissue as tester and its adjacent nonmalignant lung tissue as driver, we generated the FSL which represented up-regulated transcripts.
The E library was obtained using the cDNA of K59 (embryogenic) and C15 (non-embryogenic) genotypes as 'tester' and 'driver', respectively.
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Transgenic VAPB mice were generated using the cDNAs of wild-type or P56S-mutant human VAPB cloned into the Thy1.2-expression module.
We used the cDNA of the longest human tau isoform (2N4R isoform, 441aa) N-terminally fused to the SOS protein as a bait to screen a cDNA library constructed from human fetal brain (Fig. 1A).
In this present study, an attempt has been made to describe the toxin profile of A. labialis from Kangaroo Island using the cDNA library of its venom glands.
BLAST searches using the cDNA sequence of the human UCN3 gene (NM_053049) as a reference retrieved three bovine orthologous cDNA sequences from the GenBank database.
Sequence reads were assembled and mapped using the cDNA option of GS DeNovoAssembler (Newbler) 2.5.3 (Roche).
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