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There were some novel benefits to using the CatWalk method in the present study.
However, gait analysis using the CatWalk method has not been explored in the NP model.
First, it was possible to observe the behavioral change in the early phase using the CatWalk method.
We aimed to measure mechanical withdrawal thresholds and analyze gait patterns using the CatWalk method for the evaluation of the pain-related behavior caused by NP application.
Measurements of mechanical withdrawal thresholds were performed using von Frey filaments touching the left footpads, and gait analysis was performed using the CatWalk method.
Furthermore, using the CatWalk method, there were significant differences in stand, step cycle, duty cycle, dual stance, stand index, mean intensity, and phase dispersion at days 7 and/or 14.
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To assess sensorimotor functional recovery, we used the CatWalk method for both static and dynamic gait analysis [ 14].
We have previously observed that higher doses of indomethacin (10 mg/kg and above) resulted in even load distribution between the arthritic right hind limb and the non-arthritic left hind limb in mice with LPS-induced arthritis similar to control animals without monoarthritis, using the Catwalk gait analysis method [ 10].
Gait parameters of freely moving mice were measured using the Catwalk gait analysis system (Noldus Information Technology, The Netherlands) as described previously [ 7, 15].
Paw pressure intensity and print areas of freely moving animals were measured using the Catwalk gait analysis system (Noldus Information Technology, The Netherlands) as described previously [ 10, 37].
Further studies aimed to investigate the effects of acupuncture at different stages during stroke using the CatWalk system are required.
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