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The normalization of the signals using the capture efficiency of each of the fragments also increases the 'quantification', although it should be noted these are all relative numbers rather than a real quantification because a number of parameters cannot be controlled or assessed properly.
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The effect of a rotating process on cell capture was investigated, and the capture efficiency was demonstrated using blood samples from healthy donors spiked with human non-small cell lung cancer NCI-H16500) and breast cancer (MCF-7) cells.
The capture efficiency was inspected using a microscope to remove samples from the analysis with more than one cell captured.
Using this combined approach we i) evaluate the capture efficiency for B. burgdorferi genomes in different multiplexed capture strategies, ii) generate 30 B. burgdorferi WGS at high coverage, and iii) identify high quality genome-wide SNPs.
The capture efficiency of the multifunctional surfaces was then finally validated using cancer cell-spiked human blood.
Using these hamster-baited traps alone, we were not able to measure directly the capture efficiency of our traps.
However, the capture efficiency is also likely influenced by the number of probes used per region and the mismatched residues between the probes and their targets.
The capture efficiency of two magnetic core sizes is compared.
From this the capture efficiency can be derived.
Following elution, the capture efficiency was evaluated via q-PCR reactions.
The capture efficiency is measured by the capture rate constant.
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