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dN/dS values were determined for all species using the basic model (Model 0) and for specific lineages (Felidae and each Carnivora family) using the branch model (Model 2).
Molecular evolution of the N terminus was analyzed by PAML 4.7 [ 27], using the branch model.
We then computed the pairwise dN/dS ratios for each gene using the branch model in PAML (v. 4.6).
We tested for evidence of accelerated evolution after the retroposition event using the branch model of PAML [ 16].
For this purpose we performed an analysis of ω for each branch of the tree (using the branch model (model=1) in PAML, Supp.
Evolutionary rates of particular branches of interest (ω1) versus the background ratio (ω0) were computed using the branch model (model = 2).
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Different rates of d N/d S for branches in the phylogenetic tree were investigated using the branch models from Codeml.
First, we tested whether the evolutionary rates of each gene in the pathway differed among lineages within the gene tree by using the branch models.
We first tested whether the average ω ratio differed among lineages of the gene tree by using the branch models that allow ω to vary among lineages and assume different ω ratios assigned to the branches before and after the duplication event.
We then used the branch model to conduct pairwise comparisons among any two subcellular categories.
To identify genes with higher dN/dS ratios in branches of interest, we used the branch model in Codeml [ 48].
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