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We mapped each sequencing tag to the S. cerevisiae genome's 2006 release [55] using the bowtie tool [56], [57].
For gene expression analysis RNA-seq reads obtained for the three biological replicates for each tissue were mapped to the LAGI02 transcriptome using the Bowtie tool [ 77].
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Reads were then mapped to the 64 bp reference sequences using the Bowtie mapping tool (version 0.12.7, [ 24]).
In total, 461 M categorized reads from the GPMx mapping population were mapped to the 64 bp reference sequences using the Bowtie mapping tool [ 24].
To accomplish this, we first align the reads to the transcript sequences using the Bowtie alignment tool (Langmead et al., 2009), preserving possible multiple alignments to different transcripts.
To gain an overview of small RNA distribution in the genome, we mapped small RNAs using the Bowtie alignment tool [ 23].
SSPACE maps the paired 500-bp-insert-size library reads on the assembled contigs using the Bowtie aligner (version 0.12.5) (25) as the mapping tool.
After cleaning the data, we mapped them on the human genome reference sequence version hg19 using a mapping tool of Avadis NGS (Agilent Technologies, Santa Clara, CA, USA) or using the Bowtie program.
Sequences of the form N20-NGG in the assigned region were first extracted using the Bowtie software (version 0.12.8).
We used the Bowtie alignment tool [ 23] to filter out reads that mapped to structural RNAs: tRNA (1,640 reads), rRNA (3,456 reads) and repetitive elements (EhSINEs, EhLINEs and EhERE elements) (8,073 reads).
The Bowtie tool was used to map the reads to the annotated R. etli CFN42 genome (see Methods).
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