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Reads were then aligned against the T. reesei genome (version 2 from the JGI website) using the Bowtie mapper (version 0.12.7) [ 54].
Reads were mapped to the longest transcripts for each of the 'Oktoberfest' gene models using the bowtie mapper (version 0.12.7; '-a -v 2' options were applied) (Langmead et al., 2009).
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Deconvoluted quality-checked sequence reads were aligned to the B. taurus reference genome (Btau 4.0.63 genome release) with the TopHat splice junction mapper [version 1.3.0] [ 74, 75], which aligns reads using the Bowtie aligner (version 0.12.7).
Sequences of the form N20-NGG in the assigned region were first extracted using the Bowtie software (version 0.12.8).
This maps the reads back to the assembled transcripts using the bowtie aligner.
MiRDeep2 uses the Bowtie read mapper internally to map letter space data (Illumina) to genome sequences (Langmead et al. 2009).
TopHat internally uses the Bowtie aligner.
Using the KEGG mapper (http://www.genome.jp/kegg/mapper.html), a mapped overview of the primary human metabolic pathways demonstrated 49 mapped metabolites (Supporting Information Figure S-14).
Fluorescent PCR products were run on the Genetic Analyzer and analyzed using the Gene Mapper software.
Raw data were processed using the Gene Mapper Software 4.0 (Applied Biosystems).
Change in S/N compared with using the standard bowtie extraction region.
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