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Sequences obtained were compared with those from other Rickettsia species in GenBank by using the BLAST utility (National Center for Biotechnology Information, Bethesda, MD) and FASTA routine from GCG environment.
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A specie determination using the blast analysis was not possible.
Distinct unigenes were indentified using the BLAST search program.
All sequences were blasted using the BLAST tool of VectorBase (http://www.vectorbase.org/Tools/BLAST) against the An.
Blast searches were performed using the blast program available at http://blast.ncbi.nlm.nih.gov.nih.gov
We did the BLAST jobs on 180 nodes of the CBSU clusters (http://cbsuapps.tc.cornell.edu/index.aspx) using the P-BLAST utility.
The graphical user interface of the blast utility uses the standard bioperl Bio::GMOD::Blast::Graph package.
BLAST-based generation of common cis-regulatory elements Sequences obtained from TAIR [ 98] (1000 bp 5′ of the transcription start site) were incorporated into a local BLAST [ 103, 104] database using the formatdb utility that is included in NCBI's package download of BLAST.
Zebrafish orthologs were predicted by reciprocal BLAST analysis using the Ensembl utilities at http://www.ensembl.org.org
Edited using the MATLAB utility "GUIDE".
Estimates of utilities were based on actual patients' utilities using the health utility index [ 27].
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