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2. Sequence curation: Curators can review/edit trimmed ESTs as well as delete and submit the individual sequence to GenBank. 3. Automated BLAST: Sequences automatically undergo a BLAST against NCBI's GenBank using the BLAST tool.
This was confirmed by the high BLAST scores obtained when all fragments were searched against the sorghum genome sequence database (Phytozome) using the BLAST tool.
In addition to these fragments, an EST (Genbank Acc. No. GW403182.1) was derived from the NCBI database by using the BLAST tool (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi
All sequences were blasted using the BLAST tool of VectorBase (http://www.vectorbase.org/Tools/BLAST) against the An.
The selected ITS2 sequences were filtered then with a HMM-based annotation [35] and fungal nrITS database (http://www.emerencia.org/fungalitspipeline.html) [36] using the BLAST tool.
Primers were designed on the basis of known sequence information of Nipponbare genome (TIGRv3) by using the blast tool of Gramene (www.gramene.org).org
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Entry clones sequences were annotated based on the reference sequences using the Blast tools of the HUSAR software package [ 41].
We also used the Blast tools and resources at NCBI [ 64] and the comparative genomics platforms STRING [ 65] for additional gene clustering analysis tools.
Phylogenetic analysis was performed using the following general pipeline: homologous sequences were searched in databases using the BLAST EXPLORER tool [ 43].
TGs are also identified using only the BLAST tool, the first step in this TG mapping method, producing set TGbl.
We found this to be an error of default (discussed further below) prompting us to repeat the alignments using the BLAST-P tool at the Swiss Institute of Biotechnology SIBB).
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using the blast similarity
using the measurement tool
using the blast function
using the blast database
using the blast algorithm
using the design tool
using the construction tool
using the blast programme
using the engine tool
using the software tool
using the blast package
using the blast cleaning
using the blast search
using the blast program
using the brush tool
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