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Similarity searches were carried out using the BLAST suite [ 56].
Searching the zebrafish genome and expressed mRNAs was conducted using the BLAST suite of programs [ 47].
Homology searches for nucleotide and amino acid sequences were performed using the BLAST suite of programs (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi
Identification of rice genes homologous to GH1 β-glucosidase genes was done using the BLAST suite of programs [ 58] in 4 databases: GenBank at NCBI [ 59], the Monsanto Rice Genome Draft Database [ 60], the Beijing Genomic Institute, BGI [ 26] and the Syngenta Torrey Mesa Research Institute database [ 61].
Similar(56)
Blast searches were performed using the blast program available at http://blast.ncbi.nlm.nih.gov.nih.gov
Nucleotide and amino acid sequence alignments were analyzed using the Basic Local Alignment Search Tool (BLAST) suite of programs (http://www.ncbi.nlm.nih.gov/BLAST.cgi).nih.gov/BLAST.cgi
Sequence homology searches were performed using the standalone BLAST suite against D. melanogaster sequence databases (release 5.4) available in the FlyBase repository [ 66].
To assess the quality of the transcriptomes, a second BlastX search was performed against the TAIR10 Arabidopsis thaliana proteins (ftp://ftp.arabidopsis.org/home/tair/Proteins/TAIR10_protein_lists/TAIR10_pep_20101214, last accessed January 5, 2013; Swarbreck et al. 2008) using the BLAST+ suite (version: BLAST2.2.25+; Camacho et al. 2009) with default length and e-value settings.
Annotation against the CDD database (http://www.ncbi.nlm.nih.gov) was done using the rpsblast programe of the blast suite, and pfam using the hmmer v 3 programe.
We used the NCBI BLAST suite (BLAST 2.2.28, [ 35]) for our protein searches.
We identified mitochondrial contigs and scaffolds within ace63 and ace75 assemblies by performing similarity searches with the BLAST suite of programs (Altschul et al. 1997) using L. thermotolerans mitochondrial gene sequences as the query (Talla et al. 2005).
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