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Homology of the deduced P5CS protein with other members of this family was verified by querying the NCBI database using the BLAST software.
The sequence of the ITS region of the fungal strain VP4 showed a 100%% identity with Aspergillus sydowii species using the Blast software.
The DNA sequences were determined and analysed as previously reported [15], and compared to the GenBank nucleotide data library using the Blast software at the National Centre of Biotechnology Information website (http://www.ncbi.nlm.nih.gov/Blast.cgi) [16].
Of these genes, a total of 667 complete orthologous genes were found using the Blast software in the R. slovaca and R. akari genomes [49].
To identify mutations in the mucA gene, the sequences obtained from the different mucoid variants were analyzed for homologies with the mucA (PA0763) sequence annotated in the P. aeruginosa Genome Project [57], by using the BLAST software [58].
Each A. dehalogenans strain 2CP-C protein sequence was then compared against this database using the BLAST software package [94] using a cutoff E value of 1×10−5 and default settings for all other parameters.
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Entry clones sequences were annotated based on the reference sequences using the Blast tools of the HUSAR software package [ 41].
The primers used for HRGβ1 were designed manually, and the specificity was checked with the EMBL-GenBank database software using the Blast program.
Proteins from all genomes were blasted with the Arabidopsis protein queries (Additional file 3: Table S1) using the standalone BLAST software package.
The amino acid sequence was aligned using the protein blast software (http://blast.ncbi.nlm.nih.gov/Blast.cgi) and the DNAMAN 6.0.40 (LynnonBiosoft) software (http://lynnon-biosoft-dnaman-eng.software.informer.com/).informer.com/
The predicted genes were annotated by first comparing them to the HK1651 annotation using the NCBI BLAST software [ 46].
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