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Sequence alignment using the BLAST similarity search in the GenBank database revealed that the amino acid sequence was similar to ALS derived from Anabaena sp. PCC 7120.
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(I) Sequence similarity: Using the BLAST algorithm, the similarity on both the DNA and protein level is highest to KIR. (II) Gene structure similarity: The nine exon gene structure and the phases of the splice site boundaries are identical or more similar to KIR than to any other gene family.
Our method was also compared to one based on global sequence similarity, using the BLAST alignment bitscores as illustrated in Materials and Methods.
Another query possibility is the sequence similarity search by using the BLAST algorithm [ 38] which is useful in order to discover similarities among unknown cell cycle putative genes and the database content.
Sequences were subsequently compared using the BLAST algorithm and an overall percentage similarity match calculated.
The putative gene cassettes harboured on the integrase sequences were determined through online similarity searches using the BLAST software in the National Center for Biotechnology Information website (http://www.ncbi.nlm.nih.gov/).nih.gov/
With the aim to identify a high affinity urea transporter from maize, an in-silico search was performed based on sequence similarity with AtDUR3 (At5g45380) using the BLAST algorithm on the Aramemnon plant membrane protein database (http://aramemnon.botanik.uni-koeln.de/index.ep, ARAMEMNON v. 7.0© [ 20]).
The sequence similarity was determined by using the BLAST program from the National Center for Biotechnology Information (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi
Sequence similarity searches were conducted using the Blast algorithm, vs. the non-redundant (nr, NCBI) database.
Similarity searches were carried out using the BLAST suite [ 56].
Sequences obtained were compared for similarity to sequences in GenBank by using the BLAST program (www.ncbi.nlm.nih.gov/BLAST).nih.gov/BLAST
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