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The genes scattered in this genome were annotated using the Blast service of NCBI.
The sequences obtained were arranged into contigs and identified using the BLAST service of the GenBank database [ 13].
GeneBank searches were performed using the BLAST service at the National Center for Biotechnology Information (NCBI) [ 45].
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Users can also input their own nucleotide sequences instead of gene IDs using the BLAST services (Blastn, Blastp, Blastx tBlastn and tBlastx) provided under the Tools section in BRAD V2.0.
To be sure that the detected N-terminal peptide is specific, a blast computation was performed at the SIB using the BLAST network service [ 20].
The copy number ratio of orf224 and orf222 was determined from the Ct values and E. The NCBI database was searched using the blast network service and bl2seq (http://www.ncbi.nlm.nih.gov/).nih.gov/
A database search was executed using the BLAST network service http://blast.ncbi.nlm.nih.gov/Blast.cgi with default parameters.
All amplified sequences were analyzed and compared with the GenBank-NCBI database using the BLAST network service (http://www.ncbi.nlm.nih.gov/BLAST/).nih.gov/BLAST/
Nucleotide sequences were translated into deduced amino acid sequences using CLC Sequence Viewer 7 (CLC bio, Aarhus, Denmark) and compared using the BLAST network service at the National Center for Biotechnology Information.
The orthologous sequences of human MCF (SLC25) were obtained from Swiss-Prot/TrEMBL and GenBank using the BLAST network service [ 39, 40].
tRNA genes were identified with the tRNAscan-SE program [ 59]; other genes were identified by similarity searches in GenBank at NCBI using the BLAST network service [ 60].
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