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To be sure that the detected N-terminal peptide is specific, a blast computation was performed at the SIB using the BLAST network service [ 20].
Sequences analyses with database were performed at NCBI (http://www.ncbi.nlm.nih.gov/) using the BLAST network services, and a phylogenetic tree was generated in MEG5.1 software (http://www.megasoftware.net) to test the evolutionary relationships.
The copy number ratio of orf224 and orf222 was determined from the Ct values and E. The NCBI database was searched using the blast network service and bl2seq (http://www.ncbi.nlm.nih.gov/).nih.gov/
A database search was executed using the BLAST network service http://blast.ncbi.nlm.nih.gov/Blast.cgi with default parameters.
All amplified sequences were analyzed and compared with the GenBank-NCBI database using the BLAST network service (http://www.ncbi.nlm.nih.gov/BLAST/).nih.gov/BLAST/
The orthologous sequences of human MCF (SLC25) were obtained from Swiss-Prot/TrEMBL and GenBank using the BLAST network service [ 39, 40].
Similar(51)
A specie determination using the blast analysis was not possible.
Distinct unigenes were indentified using the BLAST search program.
All sequences were blasted using the BLAST tool of VectorBase (http://www.vectorbase.org/Tools/BLAST) against the An.
Homologies were confirmed using the blast 2 sequence tool [97].
Blast searches were performed using the blast program available at http://blast.ncbi.nlm.nih.gov.nih.gov
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