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The oligonucleotides were checked for specificity using the BLAST feature of SGD.
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A specie determination using the blast analysis was not possible.
Distinct unigenes were indentified using the BLAST search program.
All sequences were blasted using the BLAST tool of VectorBase (http://www.vectorbase.org/Tools/BLAST) against the An.
Homologies were confirmed using the blast 2 sequence tool [97].
Blast searches were performed using the blast program available at http://blast.ncbi.nlm.nih.gov.nih.gov
All blast searches were done using the NCBI blast 2.2.25+ package [ 28].
Batch blast of the unique sequences was performed using the local BLAST tools that available in ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/.nih.gov/blast/executables/blast+/LATEST/
BLAST searches were performed either locally or at National Center for Biotechnology Information (NCBI) using the NCBI BLAST program.
The next step is to blast off an RFQ to the members of your long list using the automated features of GlobalSources.com to do so.
The sequences were analyzed by using the local BLAST program.
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