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Our method was also compared to one based on global sequence similarity, using the BLAST alignment bitscores as illustrated in Materials and Methods.
All sequences were compared with sequences in GenBank by using the BLAST alignment tool (www.ncbi.nlm.nih.gov/BLAST).nih.gov/BLAST
All probes were cross-checked against the human and mouse genome using the BLAST alignment tool.
The products were sequenced and the identity of the sequences was determined using the BLAST alignment system.
The primer sequences were screened to avoid homology to other loci or repetitive sequences in the genome by using the BLAST alignment tool.
Each sequence was annotated using the BLAST alignment with the lowest expected value (≤ 1 × 10-6) in those alignments where the reading frame was in the positive orientation.
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Initially, two EST pools and all Reference sequence project (Refseq) [ 21] mRNA sequences were aligned to the human genome using the BLAST like alignment tool (BLAT) [ 22].
The U133A array contains probe sets which recognise 16 S100 genes (S100A1-S100A14, S100β and S100P), for which probe sequence identity and specificity could be verified using the BLAST sequence alignment tool (http://www.ncbi.nlm.nih.gov/BLAST/).nih.gov/BLAST/
Annotated potato loci (PGSC0003DMG40*******) corresponding to the genes were obtained using the BLAST sequence alignments tool in (http://potato.plantbiology.msu.edu/integrated_searches.shtml).shtml
It is important to keep in mind that because of the different methods used in calculating the pairwise genome identities, the demarcations obtained using the BLAST-based alignment could be different from those obtained using the global alignment and would be more likely different from those determined by other algorithms using different datasets and/or different genome regions.
Furthermore, human orthologous genes were identified by aligning pig gene-associated regions against the human genome using the BLAST-like Alignment Tool [ 53].
More suggestions(15)
using the blast function
using the blast database
using the blast algorithm
using the multisequence alignment
using the interference alignment
using the blast programme
using the default alignment
using the blast package
using the blast tool
using the blast cleaning
using the blast search
using the blast program
using the translation alignment
using the blast software
using the protein alignment
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