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Alternatively, we tested the optimal weight matrices using the best expression and pathway genes as core gene sets to find alternative sets of optimal weight matrices (see Text S3).
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While many studies recommend multiple normalization genes with three to five reference genes [ 33], we performed new REST analyses and compared the results of cytokine expression using the best combination genes selected by NormFinder software and the two or three most stable genes selected by geNorm application.
Using this system, the best expression conditions of PAMO were investigated first, including different host strains, temperature as well as time and induction period for PAMO expression.
Using a microscale approach, the best expression conditions for PAMO were investigated first, including different host strains, temperature as well as time and induction period for PAMO expression.
Our results demonstrate that the best expression was achieved using the integrating vector, a finding most likely linked to a considerable instability of the replicating vectors when expressing ffluc and lux.
The best expression was obtained using the strain BL21 DE3 -RIPL, which harBL21 DE3 -RIPL,es of tRNAargU, proL, ileY, leuwhich
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