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Phenotyping was performed using the basket method.
This result might be explained by our quantification of RDR using the basket method.
T1 seedlings were transplanted into pots, and soil-surface rooting was analyzed by using the basket method.
We measured the vertical growth angle of primary roots by using the basket method (Oyanagi et al. 1993; Nakamoto and Oyanagi 1994).
Figure 2 Distribution of primary roots between vertical-angle zones in the sor1 mutant (A, B, and C) and wild-type Nipponbare (D, E, and F), grown under waterlogged conditions by using the basket method.
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In order to predict the in vivo concentration-time profile by the in vitro release data of bio-adhesive pellets, the release experiment was performed using the rotating basket method in pH 6.8 phosphate buffer.
While the depth of a root system is particularly complicated to determine, the root cone angle is reasonably easy to measure using specific phenotyping systems such as the basket method (Oyanagi et al. 1993) or the shovelomics method in the field (Trachsel et al. 2011).
RDR was evaluated quantitatively (as an index for RGA) based on the basket method that uses open stainless-steel mesh baskets (top diameter of 7.5 cm and depth of 5.0 cm; PROUD, Ushiku, Japan), as described previously (Uga 2012).
In this study, we quantified RGA by using the ratio of deep rooting (RDR) estimated by the basket method as described previously (Uga 2012).
To evaluate the soil-surface rooting phenotype of the mutant, we used four different methods: the pot method, the cup method, the glass tube method, and the basket method.
Additional file 8 Cross-sectional diagram of the basket method.
More suggestions(15)
using the electrodeposition method
using the extraction method
using the reference method
using the penalty method
using the impregnation method
using the bisection method
using the nj method
using the receptance method
using the parsimony method
using the likelihood method
using the registration method
using the beb method
using the shooting method
using the backstepping method
using the perturbation method
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