Sentence examples for using the baculovirus expression from inspiring English sources

Using the baculovirus expression system, we expressed and characterized an epoxide hydrolase from Anopheles gambiae (AgEH) that is distinct in evolutionary history from insect juvenile hormone epoxide hydrolases (JHEHs).

A recombinant H1 HA protein derived from the A/PR8 strain was expressed using the baculovirus expression system and purified using a His-Tag affinity column.

The soluble form of HA was expressed using the Baculovirus Expression Vector System (BEVS).

Recombinant Integrator subunits with an N-terminal Flag tag were expressed in Sf9 cells using the baculovirus expression system.

Drosophila ORC was recombinantly expressed in High5 cells using the baculovirus expression system.

Therefore, we decided to also express the Drosophila FDL using the Baculovirus expression system in insect cells.

This conclusion was further confirmed when we used the baculovirus expression system, which allowed for precise control of the expression (i.e. amount) of the different proteins implicated in the activity of the cyclin E and Cdk2 complexes.

To this end, five polyhistidine-tagged recombinant proteins (BTV-16 VP3, VP7, VP7, NS2 and a truncated version of VP5 (VP5-41acids acids) were expressed using the baculovirus or Escherichia coli expression system for characterization of protective activity.

However, there are significant potential drawbacks to use of the baculovirus expression system: these include the necessity for constant maintenance of baculovirus stocks, the need for fresh batch infections to be made each time the product is required, and co-purification of recombinant baculovirus or baculovirus proteins with VLPs.

Several rounds of virus amplification steps are needed for BmNPV because the virus titer from B. mori cells is lower than that of Sf-9 cells, and it is more difficult to obtain a high titer of BmNPV than Autographa californica multiple nucleopolyhedrovirus (AcMNPV), which is generally used in the baculovirus expression system.

The Bik1 open-reading frame (ORF) was amplified from S. cerevisiae genomic DNA and inserted into the vector pKL with an N-terminal His8-ZZ tag for expression using the Baculovirus system (Fitzgerald et al., 2006).