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Using the axonal marker, NCAM, and the dendritic marker, MAP2, we next evaluated the synaptic organization of glomeruli and demonstrated the presence of axonal and dendritic compartments (Supplementary Fig. S3e).
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In order to investigate further the nature of the multiple neurites observed in double-mutant cells, we used the axonal marker Tau1 to immunolabel cells isolated from the MGE and cultured for 5 DIV (Fig. 5 G H′).
Representative immunofluorescent labeling for 3 a, b, c) sera shows labeling of axons similar to that of the axonal marker RT97 (d) indicated by asterisks (top pictures).
The accuracy of this method was confirmed by post-experiment immunostaining for the axonal marker tau-1.
The axonal localization of JIP1 was confirmed by colabeling with the axonal marker Tau-1 [6].
The reactions were found to co-localize with immunoreactivities for the axonal marker betaIII-tubulin, i.e. they conformed to axonal profiles.
The axonal-restricted targeting of AnkG occurred earlier during development than that of the axonal markers Tau1 and SMI312 demonstrating that AnkG is a very early axonal marker.
Double immunostaining for the axonal markers L1 and TAG1 was performed to examine their colocalization with GFP-labeled projections.
For investigating cerebrocortical neuronal components status, 200 kDa neurofilament (NFP) was used as axonal marker.
Since we found that AnkG is initially expressed in MNs as soon as they grew one process that emerged from the spinal cord, a process that is already or will become the axon, we compared the time course of AnkG distribution with that of the most widely used axonal marker Tau1 (Dotti et al. 1987).
To investigate the role of Unc-51 in MB development, we analyzed the anatomical phenotypes of unc-51 mutants using several axonal markers.
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