Sentence examples for using the average fluorescence from inspiring English sources
(C ) Calculation of Kd for GFP-Lpd850−1250aa actin filament binding using the average fluorescence intensity of GFP-Lpd bound to phalloidin stabilized actin filaments (20% Cy5 labeled).
The fluorescence signal was normalized to its respective initial fluorescence intensity prior to photobleaching, and the FRAP curve was constructed using the average fluorescence intensities of the recovery images.
(H ) Quantification of the effect of NGF treatment on CBFβ protein levels based on experiments described in (F and G ). CBFβ protein abundance was quantified using the average fluorescence intensity of Flag immunoreactivity per cell.
Fig. 3 shows the HsdS subunit bound to DNA with the GFP chromophore placed roughly at the distances determined by FRET using the average fluorescence lifetimes (the ocr protein roughly takes the place of the DNA in the atomic model of M.EcoKI and ocr).
In the first approximation, this inherent artifact was corrected by calculating the FRET efficiency using the average fluorescence lifetime of a subset of control cells with comparable fluorescence intensities: (2) refers to the average fluorescence lifetime of a subset of 5% of the control donor-only GFP-expressing cells with fluorescence intensities most similar to cell i.
Assuming that the MS2 sites are similarly saturated with MCP GFP protein in both cases we can then use the average fluorescence per polymerase molecule calculated for the hunchback>MS2 transgene to calibrate the BAC fluorescent traces in terms of the absolute number of transcribing polymerases per fluorescent spot.
To allow for variations in dye loading, corrected confocal fluorescence data (F) were normalized using the average resting fluorescence (F0) for each cell, as measured during periods in which there was no increase in fluorescence.
The data were analyzed using the average peak fluorescence at each inhibitor concentration tested in triplicate.
Using the average rate of DCF-DA fluorescence in whole worms as proxy for ROS flux, we found a statistically significant, linear increase in ROS production with age (Figure 4A).
Cells transfected with pc22-eGFP-TgICMAP1 were assigned to high or low expression groups using three times the average fluorescence of untransfected cells as the threshold.
Image quantification was done using Image J. The line tool was used to estimate the average fluorescence per unit area in the polygons in the syncytium or depict the fluorescence across the line in wild type and mutant embryos.
