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The fragments were sequenced using the automated sequencer 3130 Genetic Analyser (Applied Biosystems, Foster City, CA).
Genotyping the TOE1 g.2171G>A SNP was performed by sequence analysis using the automated sequencer Genetic Analyzer 3500 (Genetic Analyzer 3500, Applied Biosystems by Life Technologies GmbH, Darmstadt, Germany).
Following purification of PCR products, sequencing was performed using the automated sequencer ABI 3730 (Applied Biosystems, Foster City, CA, USA).
Sequence reactions were done using the corresponding primers used for amplification, and sequencing was performed using the automated sequencer ABI Prism 3100 (Applied Biosystems, USA).
DNA sequencing of cry1Ab gene was carried out using the automated sequencer ABI PRISM 310.
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The sequences of recombinant plasmids containing each gene were verified using the Automated DNA sequencer 3730×l (Applied Biosystems), and then the recombinant plasmids were amplified using T7- and SP6-specific primers (T7: 5'-TGT AAT ACG ACT CAC TAT AGG G-3' and SP6: 5'-CTA TTT AGG TGA CAC TAT AGA Ato3') to prepare templates for labeling cRNA probes.
The amplicons (893 bp) were sequenced using an automated sequencer by the Sanger method (ABI Prism 377 sequencer; Perkin-Elmer) [ 10].
The purified sample was sequenced using an automated sequencer.
Using sequencing primers provided in the GPS-1 kit, 48 transposon fragments/PCR reaction were sequenced using an automated sequencer model ABI 3730.
The exon 9 DNA sequence was determined using an automated sequencer.
One to three clones were sequenced using the Abi prism automated sequencer (Genome Express) and the partial pol nucleotide sequences have been submitted to the GenBank database ([GenBank: FJ707538– FJ707538]).
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