Sentence examples for using the automated liquid from inspiring English sources

We concluded that there was minimal risk of cross-contamination or carryover between wells using the automated liquid handler, and all subsequent experiments combined multiple replicates of test cases, positive controls and negative controls on individual 96-well plates.

Since no carryover or contamination between wells was detected when using the automated liquid handler, individual wells of a single 96-well plate were subsequently used to replicate experimental conditions and provide positive and negative experimental controls.

Using the automated liquid handler, pipette tips were positioned 1 mm above the bottom of 96-well plates in the center of the well, and 50 µL media was aspirated and dispensed 10 times at 200 µL/s.

Culture of Mycobacterium tuberculosis complex (MTB) was performed using the automated liquid broth MGIT™ 960 method.

However, no carryover between wells or contamination was observed using the automated liquid handler described here (Fig. 1).

For both planktonic and biofilm cultures, 5 µL of suspended cells from each well of an antimicrobial activity assay plate were inoculated onto the agar surface using the liquid-level sensing capability of the automated liquid handler.

MGIT liquid medium was incubated at 37°C for at least 6 weeks using the automated BACTEC MGIT 960 (BD Diagnostic Systems, USA).

At 36 h post transfection, plates were centrifuged (1500 rpm, 8 min) and 87.5 μl of the supernatant was transferred to new 96-well plates (Corning COSTAR #3916) or 20 μl to 384-well plates (BD FALCON 353962) using the compact automated liquid handler Hydra DT (Matrix, Thermo Fisher Scientific).

Estimation with β ̂ I 1 using the automated data.

Estimation with β ̂ A 2 using the automated data.

The primary screen was performed in duplicates in 384-well plates (BD Falcon). 5 µl of each dsRNA tested (∼0.5 µg) was spotted manually at the bottom of the wells using an automated liquid dispenser (Multidrop, Thermo Labsystems) followed by the addition of 10 µl Fringe-GFP S2 cells resuspended in Schneider's Insect medium (Sigma) without serum at a concentration of 7×105 cells/ml.