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During amplification, fluorescence data were normalized and then plotted using the automated grouping functionality provided by the LightCycler® 480 II Gene Scanning Software version 1.5.0.39 and by manual editing.
Normalized, temperature-shifted melting curves carrying sequence variation were analyzed using the automated grouping functionality provided by the LightCycler480 GeneScanning 1.5 Software (Roche Diagnostics, Penzberg, Germany).
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Estimation with β ̂ I 1 using the automated data.
Estimation with β ̂ A 2 using the automated data.
Use the automated VCIS phone for bankruptcy court information.
Moreover, we wish to add more tree estimation tools besides MrBayes [ 18, 19], e.g. RAxML [ 51], and add likelihood based methods for labeling, as well as add TFBS prediction modules to enable use without Genomatix or Mapper access, automated grouping by clustering of TFBS predictions, and import of experimental (e.g. ChIP on Chip [ 50]) data.
Hemoglobin was measured using the Sysmex automated system.
PCR-products were sequenced using the ABI automated sequencing system.
The evaluation of cell viability was carried out in each group at every experimental step, using the Countess Automated Cell Counter (Life Technologies) and trypan blue.
The screenshots were used to perform the automated lumen visibility measurements using a dedicated tool.
Liquid handling before extraction was automated using the 215 Liquid Handler (Gilson Inc., Middleton, WI), and SPE was automated using the Rapid Trace SPE workstation (Zymark, Hopkinton, MA).
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