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Micrographs were recorded on a 4 × 4 k 16-bit TVIPS TemCam-F415 CCD camera, with a physical pixel size of 15 μm by using the automated data collection system AutoEMation (Lei and Frank, 2005).
Images were recorded on SO163 film (Eastman Kodak, Rochester, NY) using an FEI (Eindhoven, The Netherlands) Tecnai Polara operating at 300 kV and a nominal magnification of 59,000× under low-dose conditions (∼20 e−/Å) by using the automated data collection system AutoEMation (Lei and Frank, 2005).
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WHAT will be the focus of the automated data collection system?
The automated data collection system is to focus on patterns of communication, consumption and movement of populations.
The automated data collection through web and native app, the calculation of road access times for emergency response units, and common operational picture management are already used in day-to-day operations.
The automated data collection also allows for the potential to analyze data more frequently than the current weekly standard.
Participants will be aware of their allocation to the intervention or control group, but the automated data collection procedures protect from researcher bias during data collection.
Estimation with β ̂ I 1 using the automated data.
Estimation with β ̂ A 2 using the automated data.
Because the side views, which are essential for three-dimensional (3D) reconstruction, accounted for only 5%10%% of the total, we used the Leginon automated data collection system (Suloway et al., 2005) to obtain 6,000 CCD frames, yielding ∼60,000 side views suitable for analysis.
However, unlike micro-costing, cost accounting systems use automated data collection, which allows for the collection of data for a larger number of patients and over a longer time period.
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