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Participating laboratories, including some with limited or no previous experience in using the assay, were able to achieve a comparable sensitivity at high specificity using IA-2βA radio-binding assays, as well as good concordance in reporting results.
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Five microliters of sample were used; the assay was conducted in duplicates.
The peptides used in the assay were the same as those used for patient vaccination.
By contrast, fullerenes and activated carbon used in the assay were largely ineffective.
All chemicals used in the assay were of at least reagent grade.
All the materials used in the assay were validated endotoxin-free.
All proteins used in the assay were purified at the same time, following the same protocol.
All materials used for the assay were rendered LPS-free.
All materials used for the assay were rendered endotoxin-free.
Primer sequences used in the assay were shown in Table 1.
Media used in the assay were MHB for MSSA and MHB +2% NaCl for MRSA.
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