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Muromonab-CD3 produced an unequivocal cytokine response using the aqueous phase assay that was remarkably similar in magnitude to the solid phase assay response at 24 h.
Also, we observed a trend of lower measurement variability with extractions using the aqueous phase, so subsequent tests used this as input to the two QIAGEN kits.
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Tap water was used as the aqueous phase and model oil with a density of 0.886 g/cm3 and a viscosity of 18.6 cP at 30 °C was used as the oil phase.
V2O5 was first dissolved in a H2O2 aqueous solution and used as the aqueous phase.
Tap water was used as the aqueous phase.
Hence, 30 mM phosphate buffer was used as the aqueous phase in this study.
Tween 80 (0.5 % v/v) containing phosphate buffer (pH 7.4) solution was heated separately at the same temperature and was used as the aqueous phase.
Different amounts of ASP agents were initially added to brine and these solutions were used as the aqueous phase.
To lower the interfacial tension up to an ultralow value, low surfactant concentration system was used where the aqueous phase of the surfactant solution is about the apparent critical micelle concentration (CMC).
A solution of 57.23% sodium chloride (NaCl), 35.82% calcium chloride (CaCl2), and 7.26% magnesium chloride (MgCl2) all dissolved in distilled water at 20% (w/w) salinity was used as the aqueous phase resembling local formation brine.
In a typical synthesis process, 7.5 ml H2O2 (30%) is added into 150 ml deionized water, and then, 0.534 g V2O5 is added into the solution; the suspension was stirred at room temperature until a dark golden yellow solution was obtained and used as the aqueous phase in this process.
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