Sentence examples for using the appropriate primary from inspiring English sources

Equal amounts of protein samples were resolved by SDS-PAGE, transferred to Immobilon-P membrane (Millipore Inc ., immunoblotted using the appropriate primary and secondary antibodies, and immunoreactive bands detected by enhanced chemiluminescence.

Sections were stained using the appropriate primary (overnight) and secondary (1 h) antibodies (Table 1).

Washing to remove unbound virus was followed by detection using the appropriate primary and labeled secondary antibody.

Left and right trachea sections were stained using the appropriate primary and secondary antibodies overnight at room temperature (Table 1).

Overexpression of the estrogen receptor (ER), progesterone receptor (PgR), and HER2 were examined by immunohistochemical staining using the appropriate primary antibodies.

Immunostaining was performed using the appropriate primary and secondary antibodies, and images were acquired using an Olympus fluorescence microscope (Osaka, Japan).

For overcoming this non-smooth behavior of solutions, using the appropriate coordinate transformation the primary system is changed into a new IAEs which its solutions have better regularity.

Analysis was conducted in Stata accounting for the complex design of both surveys, using the appropriate weighting variables and primary sampling units.

As a secondary antibody, horseradish-peroxidase conjugates for rabbit IgG (Sigma), sheep IgG (Jackson), or mouse IgG (Roche) were used to detect the appropriate primary antibody.

The slides were then subjected to a series of steps according to the previous study, except for the appropriate primary antibodies used in the present study.

Equivalent amounts of cell lysate were resolved by SDS-PAGE, transferred to polyvinylidene difluoride membrane, probed with the appropriate primary antibodies and detected using HRP-linked secondary antibodies and Amersham Pharmacia Biotech ECL western blotting reagents per manufacturer's protocols.