The antisense RNA templates for in situ hybridization were generated by in vitro transcription using PCR products (using the appropriate combinations of T7, T3, and SP6 primers) from the corresponding genes, which were cloned in BSIIKS+ or pGEMT.
Cell suspensions were prepared from draining lymph nodes, as described above, and analyzed using the appropriate combinations of the following antibodies: CD4, KJ1.26, B220, CD11c, CD69, CD62L, or Y-Ae (BD Biosciences, Oxford, UK) in 100 μl of FACS buffer (PBS, 2% fetal calf serum, and 0.05% NaN3) containing Fc Block (2.4G2 hybridoma supernatant).
Using the appropriate combination, the required release profile (burst or sustained) may be achieved.
The same effect was obtained (or rescued) by using the appropriate combination of several chloride, potassium, sodium or proton translocators (Morokuma et al., 2008; Blackiston et al., 2011).
CTL clones were established from PBMCs or CTL lines by either limiting dilution or by using the appropriate antigen-specific tetramers in combination with MACS anti-PE-microbeads (Miltenyi Biotec).
In the future, treatment of hepatitis C may even be further shortened with sofosbuvir used in the appropriate combination with other DAAs (such as ledipasvir).
Then, the mixture was centrifuged at 14,000 rpm for 1 min. Cell supernatant was transferred to a new tube and stored at 4°C. 0.5 mg of Brm antibody was mixed with 0.25 ml of wet beads (use the appropriate antibody/protein A/G combination) in room temperature for 1 hr on a rolling mixer, using serum mixed with beads as control.
To show the effectiveness of VISEE, we first illustrated it through using VISEE to select the appropriate combination of features and sampling rates.
GRASP is used to search for the appropriate combination of part-dispatching rules, and MCS is used to estimate the minimum-maximal part flow for one combination of part-dispatching rules.
This review provides background information on the hazards associated with sorting of unfixed cells and the process used to arrive at the appropriate combination of facility design, instrument placement, safety equipment, and practices to be followed.
The GPs involved prepared certain provisional diagnoses and one of the team's members (RB) conducted a search on Medline (Pubmed), Embase, and The Cochrane Library, through the portal of the Healthcare Library - Clinical Gofernance Documentheion Centre of tHealthcare Trustssts of the Province of Reggio Emilia, using different appropriate combinations of the search terms.
