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Multiple loci were frequently identified with the human protein, but could often be ruled out based on a closer orthology to other genes within a gene group (using the analyses methods herein).
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The analyses methods used are listed in Table 1.
We performed these analyses using the same methods used for our salamander shotgun data.
Additionally, at baseline a resting ECG is obtained using the aforementioned methods and analyses software (MEANS).
The data were analysed using the multidimensional methods: PCA, LDA and k-NN.
Data were analysed using the statistical methods available in the Partek® Genomic Suite 6.5 (Partek Incorporated, St Louis, Missouri, USA).
The individual interviews were analysed using the same methods.
Transcriptions will be analysed using the same methods as for the interview data in study 1.
For evolutionary trees of human sequences we repeated the analyses using the maximum-likelihood method implemented in PHYML [ 40].
Participants with missing baseline data will be included in the analyses using the missing-indicator method [ 90].
Regional and seasonal analyses using the methods described in this study may offer further insight.
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